| Summary: | Rapp UGA-42 Galvo Scanner |
| Author: | Nico Stuurman |
| License: | BSD |
| Platforms: | Windows |
| Devices: | Rapp UGA-42 |
The Rapp UGA-42 is a galvanometer-based laser targeting system for photobleaching, photoactivation, and other phototargeting applications. It uses galvo mirrors to precisely position laser beams through the microscope objective to target specific locations in the specimen.
This device adapter is similar to the original Rapp adapter for the UGA-40, but is designed for the newer UGA-42 hardware.
The Micro-Manager Projector Plugin allows microscope operators to calibrate the UGA42 with the camera and to draw spots or regions of interest on an image and phototarget these areas using the UGA-42.
Features
- Multi-laser support (Laser 1 through 4
- Point-and-fire capability for single-spot targeting
- Polygon support for complex ROI targeting
- Sequence automation for programmed scanning patterns
- TTL triggering with configurable edge detection
Properties
| Property | Description |
|---|---|
| LaserPor | Selects which laser to use (Laser 1 - 4) |
| LaserIntensity | Controls analog voltage send to the laser, hence laser intensity |
| LaserType | Continuous or pulsed |
| MaxIntensity | Max Laser Intensity |
| MinIntensity | Minimum Laser Intensity |
| SpotSize | Laser spot diameter for ROI rastering calculations, relative number of unknown dimensions |
| TTLTriggerBehavior | For TTL triggering, selects Rising Edge or Falling Edge |
| TTLTriggerMode | Select “None” for software control. I have not yet tested other modes, but they should work.” |
| TickTime | The UGA 42 has an internal “clock” and operates with sequential time slots (“ticks”), This property sets the duration of a tick in microseconds. Default is 50, minimum is 40, maximum is 1000. |
Installation
The UGA-42 requires the files “obsTools.dll” and “ROE UGA-42 SDK.DLL” in the micro-manager installation directory. The code was build agains UGA-42 SDK_1139, and it can not be guaranteed that other versions will work. If you do not have these files, please contact Rapp support and request them. The Rapp UGA42 should be auto-detected. Leave the debug property set to false (setting it to true will dramatizally slow down communication with the device). This device adapter currently only supports one UGA42.
See Also
- Rapp - Original adapter for the UGA-40
- RappLasers - Lasers for use with the UGA42. You may need these to open the safety shutter of your Rapp Lasers and to set the laser into RMI-Mix controlmode.
- Projector Plugin - For interactive phototargeting