Micro-Manager provides for the precise and rapid triggering of sequences of channels and focus positions by the camera.
LED illuminators and AOTF-based laser combiners both have very fast switching times, and are thus prime candidates for hardware synchronization.
Piezo z stages move rapidly enough such that a sequence of z positions can be synchronized with a camera running at tens of frames per second.
Micro-Manager’s multi-dimensional acquisition (MDA) engine practices opportunistic triggering: that is, if a triggered sequence is possible, then triggering will be carried out. Thus the user interface for triggered MDA is exactly the same as that for non-triggered MDA.