Micro-Manager provides for the precise and rapid triggering of sequences of channels and focus positions by the camera.
Channels
LED illuminators and AOTF-based laser combiners both have very fast switching times, and are thus prime candidates for hardware synchronization.
Focal drives
Piezo z stages move rapidly enough such that a sequence of z positions can be synchronized with a camera running at tens of frames per second.
Multi-Dimensional Acquisition
Micro-Manager’s multi-dimensional acquisition (MDA) engine practices opportunistic triggering: that is, if a triggered sequence is possible, then triggering will be carried out. Thus the user interface for triggered MDA is exactly the same as that for non-triggered MDA.
Controller devices that currently support triggered sequences
- AgilentLaserCombiner – Laser channel switching
- Arduino – TTL and DAC output states
- ASIStage – ASI piezo focus drives
- ESIOImagingControllers – AOTFs (laser combiners) and piezo focus drives
- MCL NanoDrive – MadCityLabs piezo XY and Z drives
- Marzhauser (TANGO controller) – Märzhäuser piezo focus drive