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Hardware-Triggered Sequences in Micro-Manager

Micro-Manager provides for the precise and rapid triggering of sequences of channels and focus positions by the camera.


LED illuminators and AOTF-based laser combiners both have very fast switching times, and are thus prime candidates for hardware synchronization.

Focal drives

Piezo z stages move rapidly enough such that a sequence of z positions can be synchronized with a camera running at tens of frames per second.

Multi-Dimensional Acquisition

Micro-Manager’s multi-dimensional acquisition (MDA) engine practices opportunistic triggering: that is, if a triggered sequence is possible, then triggering will be carried out. Thus the user interface for triggered MDA is exactly the same as that for non-triggered MDA.

Controller devices that currently support triggered sequences