Writing image processors for Micro-Manager

This page describes Micro-Manager 1.4 usage. Micro-Manager 2.0 and later APIs differ.

Micro-Manager has an image processing pipeline. Every time the program receives an image from a camera, it is run through this pipeline, and the DataProcessors in the pipeline make modifications to the image. For example, DataProcessors can:

1. Rotate and mirror images so that they have the same orientation as images from other cameras or as the microscope eyepiece
2. Apply offset/gain correction
3. Generate overlaid color images for cameras that split their sensor across two different dichroics

et cetera. An image goes in, the DataProcessor does some work, a different image comes out.

New DataProcessors must implement the DataProcessor interface. This interface specifies three functions: process(), makeConfigurationGUI(), and dispose(). The first function is what does the actual modification of image data (and is required to be implemented); the latter two deal with creation of user interfaces for configuring the processor, and their destruction, respectively. They are optional, but if you do not implement them then your DataProcessor cannot be configured at runtime.

You can see and adjust the current image pipeline by going to the Tools -> Image Pipeline… menu option. From this dialog, all DataProcessors that Micro-Manager knows about can be seen, created, and re-ordered. To add a DataProcessor to this list, it must be registered.

There are two primary ways to register a new DataProcessor. The first is to call the registerProcessorClass function on the gui object. This function takes as arguments the “class” of your DataProcessor and a String that names it (it is this string that is displayed in the Image Pipeline’s list of known processors). The class is what you get if you access the .class field of your processor. For example, let’s say you have a DataProcessor named MyProcessor:

import mmcorej.TaggedImage;

import org.micromanager.api.DataProcessor;

import org.micromanager.acquisition.TaggedImageQueue;

import org.micromanager.util.ReportingUtils;

class MyProcessor implements DataProcessor {

  public void process() {
     try {
        TaggedImage image = poll();
        // "Poison" means the queue is empty. We just pass the poison along
        // the queue in that case. It's not a valid image so don't try to 
        // process it.
        if (image != TaggedImageQueue.POISON) {
           // ... modify the image here ...
        }
        produce(image);
     }
     catch (Exception ex) {
        // Something went wrong; at bare minimum we should log the error.
        ReportingUtils.logError(ex);
     }  
  }
  
}

This particular DataProcessor does not implement makeConfigurationGUI() and dispose(), ergo it is not configurable. To register this DataProcessor, first you should compile it and put the .class file in the mmplugins directory. Then, in your code, you would do this:

gui.registerProcessorClass(MyProcessor.class, "My Processor");

Once this is done, the new DataProcessor will show up in the Image Pipeline dialog, where the user can add and remove it from the pipeline.

Alternately, you can create an MMProcessorPlugin object. These are plugins that specifically create DataProcessors when selected from Micro-Manager’s “Plugins” menu. MMProcessorPlugins are similar to MMPlugins (they both inherit from the same MMBasePlugin interface). However, because the DataProcessor is responsible for providing its own GUI via makeConfigurationGUI, MMProcessorPlugins do not have the show() and dispose() methods that MMPlugins do. Instead, they must provide a getProcessorClass function (in addition to the functions and fields required by MMBasePlugin). For example:

import org.micromanager.api.MMProcessorPlugin

class MyProcessorPlugin implements MMProcessorPlugin {

  // How this plugin will be shown in the "Plugins" menu. 
  public String menuName = "My Processor";
  // Optional extra tooltip string that will be shown when the mouse hovers
  // over this plugin in the "Plugins" menu.
  public String tooltipDescription = "My custom plugin for processing images";

  public static Class<?> getProcessorClass() {
     return MyProcessor.class;
  }  

}

When Micro-Manager starts up, it automatically loads all of its plugins. At this point, it will detect if your plugin is an MMProcessorPlugin, and if so, it will automatically register the corresponding DataProcessor so that it can be created. Additionally, when your plugin is selected from the “Plugins” menu, Micro-Manager will:

1. Create a new DataProcessor if one does not already exist.
2. Add that DataProcessor to the image pipeline.
3. Display the configuration GUI for that processor.