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Micro-Manager at the ASCB Meeting 2011

For the fifth year we presented the Micro-Manager software in the Exhibit Hall at the annual meeting of the American Society for Cell Biology (ASCB) in Denver. Our goal was to introduce the Micro-Manager software to new users, gain feedback from current Micro-Manager users, and to demonstrate new capabilities.

To demonstrate the software we used a spinning disk confocal system (Yokogawa/Solamere) equipped with a Photometrics Evolve 512 camera, ASI XYZ and piezo Z-stage on the new ASI RAMM Microscope, multi-color laser launch and AOTF (Solamere), ESImaging micro-controller, and running on a PC computer. All of the equipment was made available to us by the respective companies (Thanks!). Please check out slide show below.

For fast image acquisition, a triggering mechanism was set-up, resulting in continuous acquisition at a rate limited only by the exposure and read-out time of the camera. The camera, AOTF and z-piezo stage were synchronized in hardware. A TTL pulse, signalling camera exposure, drove a sequence of output patterns on the ESImaing micro-controller as well as the piezo Z controller. In addition, the system used the new ASI CRISP hardware autofocus, maintaining focus even while running the Slide Explorer plugin.

<div class="video"><iframe src="https://www.youtube.com/embed/O8zdsyNoOg8" width="400" height="300" frameborder="0" webkitallowfullscreen mozallowfullscreen allowfullscreen allow="accelerometer; encrypted-media; gyroscope; picture-in-picture"></iframe></div> <font size=-1>Movie acquired during the ASCB meeting. Drosophila S2 cell expressing GFP-histone 2B and Cherry-tubulin. Images were taken at 15 second intervals. At each time point, a z-stack of 5 slices (with 2 micron intervals) was acquired. Exposure time was 50 msec, total time to acquire a single time point (10 images) was 500 msec. </font>

We were very encouraged by the interest and very positive responses both from the user community and from the industry. On the ASCB exhibit floor we noted Micro-Manager controlling equipments in the booths of Spectral, Raptor Photonics, Axiom Optics, Sutter Instrument, ThorLabs, and the BioBus. Thanks everyone for stopping by at our booth and for all the support our project is getting from the industry! A special Thanks to George Peeters (Solamere) for all the help in set up and take down.

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