Micromanager plugin for real time closed loop control of the density of photoactived molecules during a PALM measurement. Enables automated PALM imaging.
User’s guide to follow soon!
For details of the algorithm see our paper. (And if you use the plugin for published work please cite us): Seamus J. Holden, Thomas Pengo, Karin Meibom, Carmen Fernandez-Fernandez, Justine Collier, Suliana Manley. High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization. PNAS. 201313368, 2014.
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