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Difference between revisions of "Rapp"

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<tr><td>'''License:'''</td><td>LGPL</td></tr>  
 
<tr><td>'''License:'''</td><td>LGPL</td></tr>  
 
<tr><td>'''Platform:'''</td><td>Windows</td></tr>
 
<tr><td>'''Platform:'''</td><td>Windows</td></tr>
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The Rapp UGA-40 uses galvos to bounce lasers through the microscope objective and target particular spots on the specimen in the image plane. The galvos can also be rastered to illuminate a region of interest. Micro-Manager [[Projector Plugin]] allows users to draw one or more spots or regions of interest on an image of the specimen, and phototarget these areas using the UGA-40. (The Projector Plugin permits both ad hoc phototargeting and planning targeting events embedded in a multi-dimensional acquisition).
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The UGA-40 supports up to two lasers of different colors. Choose which laser to use by setting the "Laser" property to "1" or "2".
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A few UGA-40-specific properties allow fine-grained control, supplementing the generic controls presented by the Projector Plugin. These include:
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<tr><td>'''CalibrationMode'''</td><td>If On, laser is turned on regardless of targeting status</td></tr>
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<tr><td>'''TTLTriggered'''</td><td>For TTL triggering, determines whether triggering occurs on a rising or falling edge</td></tr>
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<tr><td>'''SpotSize'''</td><td>Tells the UGA-40 controller how large the measured spot size is, for help with rastering extended ROIs</td></tr>
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<tr><td>'''RasterFrequency_Hz'''</td><td>Controls the speed of ROI rastering</td></tr>
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<tr><td>'''AccuracyPercent'''</td><td>Controls how precisely an irregular ROI is filled in during rastering</td></tr>
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<tr><td>'''OnMinimumRectSize'''</td><td>Determines the smallest tile used during rastering</td></tr>
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<tr><td>'''Sequence'''</td><td>Allows a "sequence script" to be uploaded to the UGA-40 controller (rarely used)</td></tr>
 
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Revision as of 17:22, 24 October 2013

Summary:Rapp UGA-40 Phototargeting Unit
Author:Arthur Edelstein
License:LGPL
Platform:Windows

The Rapp UGA-40 uses galvos to bounce lasers through the microscope objective and target particular spots on the specimen in the image plane. The galvos can also be rastered to illuminate a region of interest. Micro-Manager Projector Plugin allows users to draw one or more spots or regions of interest on an image of the specimen, and phototarget these areas using the UGA-40. (The Projector Plugin permits both ad hoc phototargeting and planning targeting events embedded in a multi-dimensional acquisition).

The UGA-40 supports up to two lasers of different colors. Choose which laser to use by setting the "Laser" property to "1" or "2".

A few UGA-40-specific properties allow fine-grained control, supplementing the generic controls presented by the Projector Plugin. These include:

CalibrationModeIf On, laser is turned on regardless of targeting status
TTLTriggeredFor TTL triggering, determines whether triggering occurs on a rising or falling edge
SpotSizeTells the UGA-40 controller how large the measured spot size is, for help with rastering extended ROIs
RasterFrequency_HzControls the speed of ROI rastering
AccuracyPercentControls how precisely an irregular ROI is filled in during rastering
OnMinimumRectSizeDetermines the smallest tile used during rastering
SequenceAllows a "sequence script" to be uploaded to the UGA-40 controller (rarely used)
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