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Projector Plugin

Revision as of 09:26, 23 September 2013 by Arthuredelstein (Talk | contribs)

The Projector plugin provides a single graphical user interface for controlling SLMs (spatial light modulators), such as DMDs (digital mirror devices), LCOS (liquid crystal on silicon) modulators or even digital computer projectors, and also for controlling steerable-mirror (e.g. galvo-based) phototargeting devices.

Each tab is detailed below.

Point and Shoot

Single region control by clicking on the image. This toggles micro-mirrors for SLM devices, or fires a single shot for galvo devices.

Plugin projector-point and shoot.png

Setting Description
On / Off Engages Point and Shoot mode. To prevent accidental use, one has to hold down the Ctrl key and click to activate.
Spot dwell time (Does not affect pulse laser) Stops photo illumination after set time.


Multi-region control. Programs regions to be fired with Multi-Dimensional Acquisition, or fires instantly like Point and Shoot tab using "ROI ROIs now!" button.

Plugin projector-rois.png

Setting Description
Set ROI(s) Program the device with ROI(s) drawn on the selected image.
ROI Manager To add multiple 2D regions on the image, one has to use the ImageJ ROI Manager. This button is just a shortcut to that ImageJ menu.
Loop Number of times to fire ROIs. This setting differs from the device's Repetitions property by looping after visiting each region.
Spot dwell time (Does not affect pulse laser) Stops photo illumination after set time.
Run ROIs now! Fires ROIs drawn on current image.
Run ROIs in Multi-Dimensional Acquisition Fires phototargeting devices in parallel with MDA (does not affect image capture rate).
Start Frame Timepoint in MDA to fire sequence.
Repeat every __ frames Timepoint interval in MDA to again fire sequence until MDA completes or device turned off in Setup tab.


Initial calibration.

Plugin projector-setup.png

Setting Description
On / Off Toggles device control.
Calibrate Creates calibration map of phototargeting device range to the camera pixel position. Repeat for all cameras and objectives you intend to use with the phototargeting device.
Show center spot Illuminate center of SLM or galvo device.
Phototargeting channel Toggles to specified channel during phototargeting. You don't need to set this unless some hardware needs to be moved during imaging, like a dichroic and/or a shutter. If you don't see your channel listed, you must set the Core-ChannelGroup in the Property Browser to the Group containing this channel.

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