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Difference between revisions of "Photon Transfer Curve Assistant"

(Created page with "== Photon Transfer Curve Assistant == Utility to help establish camera and illumination properties. This plugin will take series of images with varying exposure times, and p...")
 
(Photon Transfer Curve Assistant)
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This plugin will take series of images with varying exposure times, and present you with the mean and standard deviation image obtained at each exposure.
 
This plugin will take series of images with varying exposure times, and present you with the mean and standard deviation image obtained at each exposure.
  
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[[Image: PTCTools.png]]
  
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Provide even, continuous illumination of your camera.  Establish the lowest exposure time possible with your camera, and establish the exposure time that just leads to saturation of your image (which will depend on illumination intensity). Decide how many exposures (i.e., different exposure times) you would like to obtain, and how many frames per exposure. 
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The plugin will then ask you to close the shutter, or prevent light of reaching the sensor in any other way possible so that it can obtain a true dark image. 
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[[Image: PTCToolsDark.png]]
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After the plugin collected dark images, it will ask you to switch the light back on. 
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[[Image: PTCToolsLight.png]]
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After all images are collected (which may take a while), an ImageJ stack with two channels, one the mean, the other channel the std.dev. at each exposure, as well as a results table that lists the exposure time, mean and standard deviation at each exposure will be shown.  The standard deviation will be very low  (< 1) if you have a stable light source, higher values indicate significant fluctuations in the light source, which will make the results harder to interpret. 
  
 
{{Listserv_Search|Photon Transfer Curve Assistant}}
 
{{Listserv_Search|Photon Transfer Curve Assistant}}
  
 
{{Documentation Sidebar}}
 
{{Documentation Sidebar}}

Revision as of 17:17, 5 November 2019

Photon Transfer Curve Assistant

Utility to help establish camera and illumination properties.

This plugin will take series of images with varying exposure times, and present you with the mean and standard deviation image obtained at each exposure.

PTCTools.png

Provide even, continuous illumination of your camera. Establish the lowest exposure time possible with your camera, and establish the exposure time that just leads to saturation of your image (which will depend on illumination intensity). Decide how many exposures (i.e., different exposure times) you would like to obtain, and how many frames per exposure.

The plugin will then ask you to close the shutter, or prevent light of reaching the sensor in any other way possible so that it can obtain a true dark image.

PTCToolsDark.png

After the plugin collected dark images, it will ask you to switch the light back on.

PTCToolsLight.png

After all images are collected (which may take a while), an ImageJ stack with two channels, one the mean, the other channel the std.dev. at each exposure, as well as a results table that lists the exposure time, mean and standard deviation at each exposure will be shown. The standard deviation will be very low (< 1) if you have a stable light source, higher values indicate significant fluctuations in the light source, which will make the results harder to interpret.

Transfer Curve Assistant Click here to search for Photon Transfer Curve Assistant on the Micro-Manager mailing list archive.

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