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Multi-Dimensional Acquisition Programming

Revision as of 20:06, 12 June 2012 by Arthuredelstein (Talk | contribs)

Multi-dimensional acquisition is an important functionality of Micro-Manager. MDA capabilities have been dramatically revised and extended between 2010-2012.


The interfaces and classes related to MDA-related components are as follows.

JSONObject: All metadata is stored in JSONObjects. Summary metadata refers to the metadata for a particular multi-dimensional acquisition, in contrast to image metadata which is attached to every image plane.

TaggedImage: A pure data object with two fields: pixels (a byte or short array) and tags (a JSONObject for the image's metadata). TaggedImages flow sequentially through a "pipeline" in Micro-Manager. A TaggedImage is born in the swig layer when the java layer calls core.getTaggedImage or similar methods. During an MDA, after this function is called, AcquisitionEngine2010 adds appropriate tags to the TaggedImage and then adds it to its output queue. DataProcessors can then modify these TaggedImages, and then the images are finally sent for display and/or storage (by default, VirtualAcquisitionDisplay and MMImageCache, respectively). There are several important tag names that are necessary for Micro-Manager to handle images correctly: ChannelIndex, FrameIndex, PositionIndex, SliceIndex, Width, Height, PixelType. All should have integer values except PixelType, which should be a string: one of GRAY8, GRAY16, RGB32, RGB64. RGB images are stored as byte or short arrays.

IAcquisitionEngine2010: This interface (created in 2010) is concerned solely with sequencing of hardware events and acquisition of images. Its most important method, run(...), accepts a single argument of type SequenceSettings and returns a BlockingQueue of TaggedImages.

AcquisitionEngine2010: This class (implemented in clojure, but usable by Java code) implements IAcquisitionEngine2010 and carries out all hardware steps necessary to produce a Multi-Dimensional data set. It runs through channels, slices, xy positions and time points according to the SequenceSettings argument of run(...) and acquires images in the correct order at the appropriate times (frames). The engine runs on its own thread so that display and saving can be carried out independently. The engine code includes an algorithm to use sequence (burst) acquisition whenever possible, either when a zero time interval is used, or when hardware triggering is set up to switch between channels or z slices. The engine takes care of hardware errors (attempting to reload devices), and may be paused or interrupted at any time. It allows extra hardware events (Runnables) to be attached to particular points in the MDA sequence (for example, allowing photobleaching events at a particular time point).

DataProcessor<TaggedImage> (abstract class): Plugins and scripts can implement their own DataProcessors. Each DataProcessor runs on its own thread and has an input and output queue: one or more TaggedImages can be received on the input queue (by the poll() call) and modified, combined, or multiplexed, and then sent out on the output queue. Dimensional indices (such as ChannelIndex) should be adjusted by the DataProcessor to ensure that each TaggedImage is display in the right place in the MDA data set. The ImageFlipper plugin shows a simple example.

ProcessorStack: This object connects up a list of active DataProcessors. It is designed to received images from the queue returned by and produces an output queue that yields processed TaggedImages.

MMImageCache (interface ImageCache) provides a way to store images either in RAM or on disk. The MMImageCache has a pluggable saving mechanism that allows different file formats to be saved (currently two are available: single TIFF and multi-page TIFF). Incoming TaggedImages are automatically indexed according to their "Index" metadata tags (ChannelIndex, FrameIndex, SliceIndex, PositionIndex) and these can be retrieved by calling getImage with the appropriate indices. The ImageCache is wired up to the display components of Micro-Manager so that display settings and comments are also stored in RAM or saved on disk whenever they are adjusted by the user.

VirtualAcquisitionDisplay: The default display class for Micro-Manager. This display module uses ImageJ windows to display multi-dimensional data sets stored in a given ImageCache. The VirtualAcquisitionDisplay object is also connected to the MetadataPanel, which shows the current metadata, comments, and channel settings. The display is designed to adapt to different numbers of channels, slices, positions and frames and show appropriate sliders and colors. In most cases, if color images or (because of DataProcessors) extra channels, slices, positions or frames are detected, the VirtualAcquisitionDisplay will adapt to show the full range of these dimensions. It expects a single height, width, and pixel type, however.

AcquisitionWrapperEngine (interface AcquisitionEngine), is the traditional acquisition engine implementing the AcquisitionEngine interface, and available in the script panel or by calling gui.getAcquisitionEngine. This wrapper sets up the TaggedImage "pipeline" (data flow) by instantiating and connecting the various components: namely the AcquisitionEngine2010, the ProcessorStack, the VirtualAcquisitionDisplay and the MMImageCache.

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