Hardware-Triggered Sequences in Micro-Manager

Micro-Manager permits the precise and rapid triggering of sequences of channels and focus positions by the camera.

Channels

LED illuminators and AOTF-based laser combiners both have very fast switching times, and are thus prime candidates for hardware synchronization.

Focal drives

Piezo z stages move rapidly enough such that a sequence of z positions can be synchronized with a camera running at tens of frames per second.

Controller Devices that support Triggered Sequences

• AgilentLaserCombiner -- Laser channel switching
• Arduino -- TTL and DAC output states
• ASIStage -- ASI piezo focus drives
• ESIOImagingControllers -- AOTFs (laser combiners) and piezo focus drives
• Marzhauser (TANGO controller) -- Marzhauser piezo focus drive

Multi-Dimensional Acquisition