Difference between revisions of "Hardware-Triggered Sequences in Micro-Manager"
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Revision as of 19:47, 28 September 2012
Micro-Manager provides for the precise and rapid triggering of sequences of channels and focus positions by the camera.
Channels
LED illuminators and AOTF-based laser combiners both have very fast switching times, and are thus prime candidates for hardware synchronization.
Focal drives
Piezo z stages move rapidly enough such that a sequence of z positions can be synchronized with a camera running at tens of frames per second.
Controller Devices that currently support Triggered Sequences
- AgilentLaserCombiner -- Laser channel switching
- Arduino -- TTL and DAC output states
- ASIStage -- ASI piezo focus drives
- ESIOImagingControllers -- AOTFs (laser combiners) and piezo focus drives
- Marzhauser (TANGO controller) -- Marzhauser piezo focus drive
Multi-Dimensional Acquisition
Micro-Manager's multi-dimensional acquisition (MDA) engine practices opportunistic triggering: that is, if a triggered sequence is possible, then triggering will be carried out. Thus the user interface for triggered MDA is exactly the same as that for non-triggered MDA.