Difference between revisions of "Hardware-Triggered Sequences in Micro-Manager"
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− | + | Micro-Manager provides for the precise and rapid triggering of sequences of channels and focus positions by the camera. | |
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===Channels=== | ===Channels=== | ||
LED illuminators and AOTF-based laser combiners both have very fast switching times, and are thus prime candidates for hardware synchronization. | LED illuminators and AOTF-based laser combiners both have very fast switching times, and are thus prime candidates for hardware synchronization. | ||
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+ | ===Focal drives=== | ||
+ | Piezo z stages move rapidly enough such that a sequence of z positions can be synchronized with a camera running at tens of frames per second. | ||
===Multi-Dimensional Acquisition=== | ===Multi-Dimensional Acquisition=== | ||
+ | Micro-Manager's multi-dimensional acquisition (MDA) engine practices opportunistic triggering: that is, if a triggered sequence is possible, then triggering will be carried out. Thus the user interface for triggered MDA is exactly the same as that for non-triggered MDA. | ||
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+ | ===Controller devices that currently support triggered sequences=== | ||
+ | * [[AgilentLaserCombiner]] -- Laser channel switching | ||
+ | * [[Arduino]] -- TTL and DAC output states | ||
+ | * [[ASIStage]] -- ASI piezo focus drives | ||
+ | * [[ESIOImagingControllers]] -- AOTFs (laser combiners) and piezo focus drives | ||
+ | * [[MCL NanoDrive]] -- MadCityLabs piezo XY and Z drives | ||
+ | * [[Marzhauser]] (TANGO controller) -- Märzhäuser piezo focus drive |
Latest revision as of 06:31, 11 April 2017
Micro-Manager provides for the precise and rapid triggering of sequences of channels and focus positions by the camera.
Channels
LED illuminators and AOTF-based laser combiners both have very fast switching times, and are thus prime candidates for hardware synchronization.
Focal drives
Piezo z stages move rapidly enough such that a sequence of z positions can be synchronized with a camera running at tens of frames per second.
Multi-Dimensional Acquisition
Micro-Manager's multi-dimensional acquisition (MDA) engine practices opportunistic triggering: that is, if a triggered sequence is possible, then triggering will be carried out. Thus the user interface for triggered MDA is exactly the same as that for non-triggered MDA.
Controller devices that currently support triggered sequences
- AgilentLaserCombiner -- Laser channel switching
- Arduino -- TTL and DAC output states
- ASIStage -- ASI piezo focus drives
- ESIOImagingControllers -- AOTFs (laser combiners) and piezo focus drives
- MCL NanoDrive -- MadCityLabs piezo XY and Z drives
- Marzhauser (TANGO controller) -- Märzhäuser piezo focus drive