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Difference between revisions of "Hardware-Triggered Sequences in Micro-Manager"

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Micro-Manager features a triggering framework that permits the precise and rapid synchronization of some hardware components with a camera.
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__NOTOC__
 
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Micro-Manager provides for the precise and rapid triggering of sequences of channels and focus positions by the camera.
===Supported Controller Devices===
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* [[Arduino]] -- Allows triggered sequencing of TTL and DAC states
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* [[ASIStage]] -- Allows triggered sequencing of ASI piezo focus drives
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* [[AgilentLaserCombiner]] -- Accepts TTL pulses for laser channel switching, as well as DAC control of piezo cofus drives
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* [[ESIOImagingControllers]] -- controls AOTFs (laser combiners) and piezo focus drives
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* [[Marzhauser]] -- triggering of focus drive
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===Channels===
 
===Channels===
 
LED illuminators and AOTF-based laser combiners both have very fast switching times, and are thus prime candidates for hardware synchronization.  
 
LED illuminators and AOTF-based laser combiners both have very fast switching times, and are thus prime candidates for hardware synchronization.  
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===Focal drives===
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Piezo z stages move rapidly enough such that a sequence of z positions can be synchronized with a camera running at tens of frames per second.
  
 
===Multi-Dimensional Acquisition===
 
===Multi-Dimensional Acquisition===
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Micro-Manager's multi-dimensional acquisition (MDA) engine practices opportunistic triggering: that is, if a triggered sequence is possible, then triggering will be carried out. Thus the user interface for triggered MDA is exactly the same as that for non-triggered MDA.
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===Controller devices that currently support triggered sequences===
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* [[AgilentLaserCombiner]] -- Laser channel switching
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* [[Arduino]] -- TTL and DAC output states
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* [[ASIStage]] -- ASI piezo focus drives
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* [[ESIOImagingControllers]] -- AOTFs (laser combiners) and piezo focus drives
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* [[MCL NanoDrive]] --  MadCityLabs piezo XY and Z drives
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* [[Marzhauser]] (TANGO controller) --  Märzhäuser piezo focus drive

Latest revision as of 07:31, 11 April 2017

Micro-Manager provides for the precise and rapid triggering of sequences of channels and focus positions by the camera.

Channels

LED illuminators and AOTF-based laser combiners both have very fast switching times, and are thus prime candidates for hardware synchronization.

Focal drives

Piezo z stages move rapidly enough such that a sequence of z positions can be synchronized with a camera running at tens of frames per second.

Multi-Dimensional Acquisition

Micro-Manager's multi-dimensional acquisition (MDA) engine practices opportunistic triggering: that is, if a triggered sequence is possible, then triggering will be carried out. Thus the user interface for triggered MDA is exactly the same as that for non-triggered MDA.

Controller devices that currently support triggered sequences

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